Alkalisk fosfatase (AP)
Engelsk navn: | Alkaline phosphatase | |
Analyseinstrument: | Advia®1800 | |
Reagensprodusent: | Siemens Medical Solutions Diagnostics | |
Analyseprinsipp: | Modifisert IFCC The alkaline phosphatase hydrolyses the PNPP substrate to form p-nitrophenol that is coloured and provides its own chromogen. The reaction is followed by the colorimetric measurement of the rate of formation of p-nitrophenol at 410 nm, which is proportional to the alkaline phosphatase activity. Metoden tatt i bruk: 24.04.05 | |
Referanse : | 1. | Alkaline Phosphatase Study Group. Committee on Standards of the AACC, Subcommittee on Enzymes, Tietz NW (chairman) et al. Progress in the development of a recommended method for alkaline phosphatase activity measurement. Clin Chem 26(7):1023 (1980) |
2. | Tietz, NW: Clinical Guide to Laboratory Tests, 3rd Edition WB Saunders Company, Philadelphia, PA pp 30-33 (1995) |