Alkalisk fosfatase (AP)

Engelsk navn:Alkaline phosphatase
Reagensprodusent:Siemens Medical Solutions Diagnostics
Analyseprinsipp:Modifisert IFCC
The alkaline phosphatase hydrolyses the PNPP substrate to form
p-nitrophenol that is coloured and provides its own chromogen. The reaction is followed by the colorimetric measurement of the rate of formation of p-nitrophenol at 410 nm, which is proportional to the alkaline phosphatase activity.
Metoden tatt i bruk: 24.04.05
Referanse :1.Alkaline Phosphatase Study Group. Committee on Standards of the AACC, Subcommittee on Enzymes, Tietz NW (chairman) et al. Progress in the development of a recommended method for alkaline phosphatase activity measurement. Clin Chem 26(7):1023 (1980)
2.Tietz, NW: Clinical Guide to Laboratory Tests, 3rd Edition
WB Saunders Company, Philadelphia, PA pp 30-33 (1995)