Amylase (Amy)

Engelsk navn:Amylase
Analyseinstrument:Advia®1800
Reagensprodusent:Siemens Medical Solutions Diagnostics
Analyseprinsipp:Ethylidene Blocked-pNPG7
This rate method uses ethylidene blocked p-nitrophenyl-maltoheptaoside as substrate. The indicator enzyme α-glucosidase, used to release the p-nitrophenol, is also employed in the method. The terminal glucose of the substrate is chemically blocked preventing cleavage by the indicator enzymes and is measured at 410 nm.
Metoden tatt i bruk: 01.07.01
Referanse :1.Jensen AP and Wydeveld A: α-(p-nitrophenyl)malto hexaoside as a substrate for the assay of amylase.
Nature 182:525-526 (1958)
2.Tietz, NW: Clinical Guide to Laboratory Tests, 3rd Edition
WB Saunders Company, Philadelphia, PA pp 46-49 (1995)
3.Tietz, NW: Clinical Guide to Laboratory Tests, 2nd Edition
WB Saunders Company, Philadelphia, PA pp 52 (1990).