B-hydroksybutarat (HBA)

Engelsk navn:ß-hydroxybutarate
Analyseprinsipp:Enzymatic / NAD+ – NADH
This kinetic enzymatic method is based on the oxidation of ß-hydroxybutarate to acetoacetate by the enzyme ß-hydroxybutarate dehydrogenase. Concomitant with this oxidation the cofactor NAD+ is reduced to NADH. The change in absorbance at 340 nm can be directly correlated with the ß-hydroxybutyrate concentration.(Metoden tatt i bruk: 1/7-01)