Bilirubin, direkte (Dbili, konjungert bilirubin)

Engelsk navn:Direct bilirubin (conjugated bilirubin)
Reagensprodusent:Siemens Medical Solutions Diagnostics
Analyseprinsipp:Vanadate oxidation
The bilirubin is oxidized by vanadate at about pH 3 to produce biliverdin. In the presence of detergent and vanadate, conjugated (direct) bilirubin is oxidized. This oxidation reaction causes a decrease in the optical density of the yellow color, which is specific to bilirubin. The decrease in optical density at 451/545 nm is proportional to the direct bilirubin concentration in the sample. The concentration is measured as an endpoint reaction.(Metoden tatt i bruk: 15/8-07)
Referanse :1.Tokuda K, Tanimoto K. New method of measuring serum bilirubin using vanadic acid. Jpn J Clin Chem. 1993;22:116-122.
2.Tietz Fundamentals of Clinical Chemistry. 5th ed. Burtis CA, Ashwood ER, eds. Philadelphia, PA: WB Saunders Company; 2001:605.
3.Young DS. Effects of Drugs on Clinical Laboratory Tests. 3rd ed. Washington: AACC Press (1990).
4.Clinical and Laboratory Standards Institute (formerly NCCLS). Evaluation of Precision Performance of Quantitative Measurement Methods; Approved Guideline – Second Edition. Wayne, PA: Clinical and Laboratory Standards Institute; 2004. NCCLS Document EP05-A2.
5.Tietz NW. Clinical Guide to Laboratory Tests. 3rd ed. Philadelphia, PA: WB Saunders Company; 1995:88-91.
6.Study Group on Bilirubin for the Committee on Standards of the American
Association for Clinical Chemistry and the National Reference System for the Clinical Laboratory. NCCLS; Dec. 1986.