Bilirubin, total (Tbili)

Engelsk navn:Total bilirubin
Reagensprodusent:Siemens Medical Solutions Diagnostics
Analyseprinsipp:Vanadate oxidation The bilirubin is oxidized by vanadate at about pH 3 to produce biliverdin. In the presence of the detergent and the vanadate, both conjugated (direct) and unconjugated bilirubin are oxidized. This oxidation reaction causes the decrease in the optical density of the yellow color, which is specific to bilirubin. The decrease in optical density at 451 nm is proportional to the total bilirubin concentration in the sample.(Metoden tatt i bruk: 5/1-06)
Referanse :1.Tokuda K. Tanimoto K. New method of measuring serum bilirubin using vanadic acid. Jpn J Clin. Chem. 1993:22(2);116-122.
2.Tietz NW. Fundamentals of Clinical Chemistry, 5th ed. Edited by Burtis CA. and Ashwood ER. WB Saunders Company; 2001: 605
3.Young DS. Pestaner LC, Gibberman V. Effect of drugs on clinical laboratory tests. Clin. Chem. 1975:21(5):1D-432D.
4.Tietz NW. Clinical Guide to Laboratory Tests, 3rd ed. Philadelphia: WB Saunders Company; 1995: 88-91.
5.National Committee for Clinical Laboratory Standards. Precision performance of clinical chemistry devices; Approved Guideline- Second Edition. Wayne, PA: NCCLS; 1999. NCCLS Document EP5-A.
6.Study Group on Bilirubin for the Committee on Standards of the American Association for Clinical