Xylose

Engelsk navn: Xylose Analyseinstrument: Pharmacia Spectrophotometer, manuell metode Reagensprodusent: Sentrallaboratoriet Analyseprinsipp: When heated with acid, xylose is dehydrated to furfural, witch in turn reacts with p-bromaniline. A pink color is produced in this reaction and measured at 540 nm. Referanse: Clin. Chem. 25, 1440-1443, 1979.

Urin, total protein (U-protein)

Engelsk navn: Urine, total protein Analyseinstrument: ADVIA®1800 Reagensprodusent: Siemens Healthcare Diagnostics Inc. Analyseprinsipp: This method is an endpoint chemistry. It is based on the measurement of the shift in absorption that occurs when pyrogallol red-molybdate complex binds with protonated basic amino groups of proteins at an acidic pH. The increase in absorbance at 596/694 nm…

Urinsyre (UA)

Engelsk navn: Uric Acid Analyseinstrument: ADVIA®1800 Reagensprodusent: Siemens Healthcare Diagnostics Inc. Analyseprinsipp: Enzymatic/Trinder The uric acid is converted by uricase to allantoin and hydrogen peroxide. A coloured complex is formed from hydrogen peroxide, 4-aminophenazone and TOOS [N-ethyl-N-(2-hydroxy-3-sulfopropyl)-3-methylaniline] under the catalytic influence of peroxidase. The abosorbance of the complex is measured as an endpoint reaction at…

Urea (Urea)

Engelsk navn: Urea Nitrogen Analyseinstrument: Advia®1800 Reagensprodusent: Siemens Medical Solutions Diagnostics Analyseprinsipp: Enzymatic: urease with GLDH The urea is hydrolysed in the presence of water and urease to produce ammonia and carbon dioxide. The ammonia reacts with 2-oxoglutarate in the presence of glutamate dehydrogenase and NADH. The oxidation of NADH to NAD is measured as…

Tyroksin, fritt (FT4, fritt T4) (hund)

Engelsk navn: Free T4, (Canine) Analyseinstrument: IMMULITE® 2000 Reagensprodusent: Siemens Medical Solutions Diagnostics Analyseprinsipp: Free T4 is a solid-phase, chemiluminescent, competitive analog immunoassay.For detailed description: IMMULITE® 2000; Principles of operation  Free T4 procedure is a direct or single test assay, in the sense that its results are not calculated as a function of total T4,…

Tyroksin, total (TT4, total T4)

Engelsk navn: Total T4 Analyseinstrument: IMMULITE® 2000 Reagensprodusent: Siemens Medical Solutions Diagnostics Analyseprinsipp: Total T4 is a solid-phase, chemiluminescent competitive immunoassay. For detailed description: IMMULITE® 2000; Principles of operation(Metoden tatt i bruk: 8/3-04) Referanse: 1. lshaw BE, Rijnberk A. Radioimmunoassay of plasma T4 and T3 in the diagnosis of primary hypothyroidism in dogs. J Am…

Tyreoglobulin antistoffer (TgAA)

Engelsk navn: Thyroglobulin Autoantibody Analyseinstrument: Tecan Sunrise Absorbance Reader Reagensprodusent: Oxford Laboratories, Inc Analyseprinsipp: This indirect enzyme immunoassay is for the determination of Tg autoantibodies in canine serum. Purified canine Tg is coated on the wells of a 96-well polystyrene plate. Canine test sera, along with positive and negative canine control sera are added to…

Triglyserider (Tg)

Engelsk navn: Triglycerides Analyseinstrument: Advia®1800 Reagensprodusent: Siemens Medical Solutions Diagnostics Analyseprinsipp: Enzymatic/Trinder without serum blank The triglycerides are converted to glycerol and free fatty acids by lipoprotein lipase. The glycerol is then converted to glycerol-3-phosphate by glycerol kinase in the presence of glycerol-3-phosphate-oxidase to form hydrogen peroxidase. A coloured complex is formed from hydrogen peroxide,…

Sink(Zn)

Engelsk navn: Zinc Analyseinstrument: Advia®1800 Reagensprodusent: Randox Analyseprinsipp: After deproteinizing zinc forms a red chelate with 5-Br-PAPS (2-[(5-Bromo-2-pyridylazo]-5-[N-propyl-N-(3-sulfopropyl)amino]phenol) at pH 7.5-9.5  The formation is measured at 545 nm. (Metoden tatt i bruk: 1/7-01) Referanse: 1. M. Saito, T. Makino et al., Clinica Chimica Acta, 120 (1982) 127-135. 2. R. Homster, B. Zak, Clin. Chem. 31/8,…

SAA (Serum Amyloid A)

Engelsk navn: Serum Amyloid A Analyseinstrument: Advia®1800 Reagensprodusent: Eiken Chemical Co., Tokyo, Japan Analyseprinsipp: Anti SAA coated latex agglutination photometric immunoassay. SAA is a sensitive and rapid acute phase reactant for the monitoring of inflammatory diseases and assessing the prognosis. (Metoden tatt i bruk: Januar 2009) Referanse: 1. Jacobsen S, Kjelgaard-Hansen M, Hagbard Petersen H,…