Protein, total (Tprot)

Engelsk navn: Total Protein Analyseinstrument: Advia®1800 Reagensprodusent: Siemens Medical Solutions Diagnostics Analyseprinsipp: Biuret Protein peptide bonds interact with cupric ions to form a purple complex which is measured as an endpoint reaction at 545 nm. (Metoden tatt i bruk: 25/11-02) Referanse: 1. Weichselbaum TE : An accurate and rapid method for the determination of proteins…

Progesteron (Prog)

Engelsk navn: Progesteron Analyseinstrument: IMMULITE®2000 Reagensprodusent: Siemens Medical Solutions Diagnostics Analyseprinsipp: Progesterone is a solidphase, competitive chemiluminescent enzyme immunoassay. For detailed description: IMMULITE® 2000; Principles of operation (Metoden tatt i bruk: 8/3-04) Referanse: 1. Aufrere MB, Benson H. Progesterone: an overview and recent advances. JPharm Sci 1976; 65:783-800. 2. Bauman J. Basal body temperature: unreliable…

Osmolalitet (Osmol)

Engelsk navn: Osmolality Analyseinstrument: Fiske 110 Osmometer Reagensprodusent: Fiske®Associates Analyseprinsipp: Freezing point osmometry measures the total solute concentration in a liquid. It is most convenient to describe freezing point as the concentration of particles in solution. Freezing point won’t tell you how big these particles are, or what shape they have, or if they are…

Natrium (Na)

Engelsk navn: Sodium Analyseinstrument: Advia®1800 Reagensprodusent: Siemens Medical Solutions Diagnostics Analyseprinsipp: Ion Selective Electrode (ISE), diluted This method is based on an indirect potentiometric procedure using an ion selective electrode. The sodium ion selective electrode responds selectively to sodium ions according to the Nernst equation. The sample is mixed with ISE buffer, thereby providing a…

Magnesium (Mg)

Engelsk navn: Magnesium Analyseinstrument: Advia®1800 Reagensprodusent: Siemens Medical Solutions Diagnostics Analyseprinsipp: Xylidyl Blue Magnesium ions reacts with xylidyl blue in an alkaline medium to form a water soluble purple-red chelate. The increase in absorbance of xylidyl blue at 505 nm is proportional to the concentration of magnesium in the sample. (Metoden tatt i bruk: 1/7-01)…

Lipase (Lip)

Engelsk navn: Lipase Analyseinstrument: Advia®1800 Reagensprodusent: Siemens Medical Solutions Diagnostics Analyseprinsipp: Colorimetric rate The chromogenic lipase substrate, DGGMR, (1,2-o-dilauryl-rac-glycero-3-glutaric acid-(6′-methylresorufin) ester) is cleaved by the catalytic action of lipase to form 1,2-o-dilauryl-rac-glycerol and an unstable intermediate, glutaric acid-(6′-methyl resorufin) ester. This decomposes spontaneously in an alkaline solution to form glutaric acid and methylresorufin. The lipase…

Laktat dehydrogenase (LD)

Engelsk navn: Lactate Dehydrogenase Analyseinstrument: Advia®1800 Reagensprodusent: Siemens Medical Solutions Diagnostics Analyseprinsipp: Enzymatio (L-P) / TRIS / NAD+-NADH LD catalyses the conversion of L-Lactate to pyruvate in the presence of NAD (nicotinamide adenine dinucleotide). The enzymatic activity of LD is proportional to the rate of production of NADH (reduced NAD). The amount of NADH produced…

Laktat (Lakt)

Engelsk navn: Lactate Analyseinstrument: Advia®1800 Reagensprodusent: Roche Analyseprinsipp: Enzymatic/Trinder First, lactate is converted to pyruvate in an enzymatic reaction. The hydrogen peroxide produced by this reaction is then used in an enzymatic reaction to generate a coloured dye. The intensity of the colour formed is proportional to the L-lactate concentration and is measured as an…

Kreatinin (Kreat)

Engelsk navn: Creatinine Analyseinstrument: Advia®1800 Reagensprodusent: Siemens Medical Solutions Diagnostics Analyseprinsipp: Jaffe, kinetic The creatinine reacts with the alkaline picric acid and forms a coloured complex. The rate of complex formation is measured at 505 nm and is proportional to the creatinine concentration. (Metoden tatt i bruk: 1/7-01) Referanse: 1. Jaffe MZ: Über den Niederschlag,…

Kreatin Kinase (CK)

Engelsk navn: Creatine Kinase Analyseinstrument: Advia®1800 Reagensprodusent: Siemens Medical Solutions Diagnostics Analyseprinsipp: NAC activated (DGKC) Creatine Kinase reacts with creatine phosphate and ADP to form ATP which is coupled to the hexokinase-GPD reaction, generating NADPH. The concentration of NADPH is measured by the increase in absorbance at 340 nm. (Metoden tatt i bruk: 1/7-01) Referanse:…