Category Archives: Metoder

Frie fettsyrer (FFS)

Engelsk navn:Non-esterified (or free) fatty acids
Analyseinstrument:Advia®1800
Reagensprodusent:
Analyseprinsipp:Enzymatic – colorimetric
This enzymatic method relies upon the acylation of coenzyme A(CoA) by the fatty acids in the presence of added acyl-CoA synthetase (ACS). The acyl-CoA produced is oxidized by added acyl-CoA oxidase (ACOD) with the generation of hydrogen peroxide. Hydrogen peroxide, in the presence of peroxidase (POD) permits the oxidative condensation of 3-methyl-N-ethyl-N-(b-hydroxyethyl)-aniline (MEHA) with 4-aminoantipyrine to form a purple color which can be measured at 545nm.(Metoden tatt i bruk: 1/7-01)
Referanse :1.Krebs, m. et al., Prevention of in Viro Lipolysis by Tetrahydrolipstatin. Clin. Chem. 46(7), 950-954 (2000)
2.Mulder, C., Schouten, J.A., and Popp-Snijders, Determination of Free Fatty Acids: A Comparative Study of the Enzymatic Versus the Gas Chromatography and Colorimetric Method. J. Clin. Chem. Clin. Biochem. 21, 823-827 (1983)
3.Aufenanger, J and Kaffermann, R. Klinisch-chemische Messgrösse: Freie Fettsäuren (FFS), S. 319-320 in Greiling/Gressner: Lehrbuch der Klinischen Chemie und Pathobiochemie. 3. edition, Schattauer 1995

Fosfor, uorganisk (P)

Engelsk navn:Inorganic phosphorus
Analyseinstrument:Advia®1800
Reagensprodusent:Siemens Medical Solutions Diagnostics
Analyseprinsipp:Phosphomolybdate/UV
Inorganic phosphorus reacts with ammonium molybdate in the presence of sulfuric acid to form an unreduced phosphomolybdate complex which is measured as an endpoint reaction at 340 nm.
(Metoden tatt i bruk: 1/7-01)
Referanse :1.Daly JA and Ertinghausen G: Direct method for determining inorganic phosphate in serum with the centrifichem.
Clin Chem 18: 263-265 (1972)
2.Tietz, NW: Clinical Guide to Laboratory Tests, 3rd Edition
WB Saunders Company, Philadelphia, PA p.486 (1995)

CRP (C-reaktivt protein)

Engelsk navn:Canine C-reactive Protein
Analyseinstrument:Advia®1800
Reagensprodusent:Randox
Analyseprinsipp :The CRP method is a polyethylene glycol (PEG) enhanced immunoturbidimetric assay. Sample is reacted with specific antiserum to form a precipitate that is measured turbidimetrically at 340 nm. By constructing a standard curve from the absorbance of standards, concentration can be determined.

(Metoden tatt i bruk: Mai 2009)

Referanse :1.Kjelgaard-Hansen M., Jensen A.L., Kristensen A.T. 2003 Evaluation of a commercially available human C-reactive protein (CRP) turbidometric immunoassay for determination of canine serum CRP concentration. Vet Clin Pathol 32:81-87.

Cortisol (Cort)

Engelsk navn:Cortisol
Analyseinstrument:IMMULITE® 2000
Reagensprodusent:Siemens Medical Solutions Diagnostics
Analyseprinsipp :IMMULITE 2000 Cortisol is a solid-phase,competitive chemiluminescent enzyme immunoassay.
For detailed description: IMMULITE® 2000; Principles of operation

(Metoden tatt i bruk: 8/3-04)

Referanse :1.Foster L, Dunn R. Single-antibody technique for radioimmunoassay of cortisol in unextracted serum or plasma. Clin Chem 1974;20:365.
2.Farmer R, Pierce C. Plasma cortisol determinations: radioimmunoassay andcompetitive protein binding compared. Clin Chem 1974; 20:411.
3.Ruder H, et al. A radioimmunoassay for cortisol in plasma and urine. J Clin Endo Metab 1972;35:219.
4.Rothfeld B, ed. Plasma Cortisol. In: Nuclear medicine in vitro. 1974:120.
5.Murphy B, et al. Clinical studies utilizing a new method for the serial determination of plasma corticoids. J Canad Med Assoc 1964;90:775.
6.Sparks R. Measurement of serum 11-deoxycortisol and cortisol after metyrapone. Ann Intern Med 1971;75:717.
7.Kowalski A, Paul W. A simple extraction procedure for the determination offree (unconjugated) cortisol in urine by radioimmunoassay. Clin Chem 1976;25:1152.
8.Burtis CA, Ashwood ER, editors. Tietz textbook of clinical chemistry. 2nd ed. Philadelphia: W.B. Saunders, 1994.
9.Wilson JD, Foster DW, editors. Williams textbook of endocrinology. 7thed. Philadelphia: W.B. Saunders, 1985

Canine TSH (TSH, tyreoideastimulerende hormon) (hund)

Engelsk navn:Canine TSH
Analyseinstrument:IMMULITE® 2000
Reagensprodusent:Siemens Medical Solutions Diagnostics
Analyseprinsipp :Canine TSH is a solidphase, two-site chemiluminescent immunometric assay.
For detailed description: IMMULITE® 2000; Principles of operation(Metoden tatt i bruk: )
Referanse :1.Ferguson DC. Thyroid function tests in the dog. Veterinary Clinics of North America: Small Animal Practice 1984;14:783-808.
2.Panciera DL. Hypothyroidism in dogs: 66 cases (1987-1992). JAVMA 1994;204:761-7.
3.Nesbitt GH, Izzo J, Peterson L, Wilkins RJ. Canine hypothyroidism: A retrospective study of 108 cases. JAVMA 1980;177:1117-22.
4.Nachreiner RF, Refsal KR. Radioimmunoassay monitoring of thyroid hormone concentration in dogs on thyroid replacement therapy: 2,674 cases (1985-1987). JAVMA 1992;201:623-9.
5.Budsberg SC, Moore GE, Klappenbach K. Thyroxineresponsive unilateral forelimb lameness and generalized neuromuscular disease in four hypothyroid dogs. JAVMA 1993;202:1859-60.
6.Beale KM. Current diagnostic techniques for evaluating thyroid function in the dog. Vet Clin North Am 1990;20:1429-41.
7.Ferguson DC. Update on diagnosis of canine hypothyroidism. Vet Clin North Am 1994;24:515-39.
8.Gonzalez E, Quadri SK. Effects of aging on the pituitarythyroid axis in the dog. Exp Gerontol 1988;23:151-60.
9.Kaptein EM, Hays MT, Ferguson DC. Thyroid hormone metabolism; a comparative evaluation. Vet Clin North Am 1994;24:431-63.
10.Kemppainen RJ, Clark TP. Etiopathogenesis of canine hypothyroidism. Vet Clin North Am 1994;24:467-76.
11.Scarlett JM. Epidemiology of thyroid diseases of dogs and cats. Vet Clin North Am 1994;24:477-86.

Canine TLI (TLI, trypsinlignende immunoreaktivitet)

Engelsk navn:Canine TLI (Trypsin-like immunoreactivity)
Analyseinstrument:IMMULITE®2000
Reagensprodusent:Siemens Medical Solutions Diagnostics
Analyseprinsipp:Canine TLI is a solidphase, enzyme-labeled, chemiluminescent immunometric assay.
For detailed description: IMMULITE® 2000; Principles of operation

(Metoden tatt i bruk: 29/9-05)

Referanse:1.Williams DA, Batt RM. Diagnosis of canine exocrine pancreatic insufficiency by the assay of serum trypsin-like immunoreactivity. J Small Anim Practice 1983;24:582-8.
2.Williams DA. New tests of pancreatic and small intestinal function. Compendium on Continuing Education for the Practicing Veterinarian 1987;9:1167-74.
3.Williams DA. Exocrine pancreatic disease. In: Ettinger SJ, editor. Textbook of veterinary internal medicine: Diseases of the dog and cat. 2nd ed. Philadelphia: W.B. Saunders, 1989: 1528-54.
4.Williams DA, Batt RM. Exocrine pancreatic insufficiency diagnosed byradioimmunoassay of serum trypsin-like immunoreactivity in a dog with a normal BT-PABA test result. J Am Anim Hosp Assoc 1986;22:671-74.
5.Williams DA, Batt RM. Sensitivity and specificity of radioimmunoasay of
serum trypsin-like immunoreactivity for the diagnosis of canine exocrine pancreatic insufficiency. J Am Anim Hosp Assoc 1988;192:195-201.
6.Williams DA. Kansas State University, personal communications.
7.Simpson KW, et al. Circulating concentrations of trypsin-like immunoreactivity and activities of lipase and amylase after pancreatic duct ligation in dogs. Am J Vet Res 1989;50:629-32.
8.Borgström A, Ohlsson K. Immunoreactive trypsins in sera from dogs before and after induction of experimental pancreatitis. Hoppe-Seyler’s Z Physiol Chem 1980;361:625-31.

Bilirubin, total (Tbili)

Engelsk navn:Total bilirubin
Analyseinstrument:Advia®1800
Reagensprodusent:Siemens Medical Solutions Diagnostics
Analyseprinsipp:Vanadate oxidation The bilirubin is oxidized by vanadate at about pH 3 to produce biliverdin. In the presence of the detergent and the vanadate, both conjugated (direct) and unconjugated bilirubin are oxidized. This oxidation reaction causes the decrease in the optical density of the yellow color, which is specific to bilirubin. The decrease in optical density at 451 nm is proportional to the total bilirubin concentration in the sample.(Metoden tatt i bruk: 5/1-06)
Referanse :1.Tokuda K. Tanimoto K. New method of measuring serum bilirubin using vanadic acid. Jpn J Clin. Chem. 1993:22(2);116-122.
2.Tietz NW. Fundamentals of Clinical Chemistry, 5th ed. Edited by Burtis CA. and Ashwood ER. WB Saunders Company; 2001: 605
3.Young DS. Pestaner LC, Gibberman V. Effect of drugs on clinical laboratory tests. Clin. Chem. 1975:21(5):1D-432D.
4.Tietz NW. Clinical Guide to Laboratory Tests, 3rd ed. Philadelphia: WB Saunders Company; 1995: 88-91.
5.National Committee for Clinical Laboratory Standards. Precision performance of clinical chemistry devices; Approved Guideline- Second Edition. Wayne, PA: NCCLS; 1999. NCCLS Document EP5-A.
6.Study Group on Bilirubin for the Committee on Standards of the American Association for Clinical

Bilirubin, direkte (Dbili, konjungert bilirubin)

Engelsk navn:Direct bilirubin (conjugated bilirubin)
Analyseinstrument:Advia®1800
Reagensprodusent:Siemens Medical Solutions Diagnostics
Analyseprinsipp:Vanadate oxidation
The bilirubin is oxidized by vanadate at about pH 3 to produce biliverdin. In the presence of detergent and vanadate, conjugated (direct) bilirubin is oxidized. This oxidation reaction causes a decrease in the optical density of the yellow color, which is specific to bilirubin. The decrease in optical density at 451/545 nm is proportional to the direct bilirubin concentration in the sample. The concentration is measured as an endpoint reaction.(Metoden tatt i bruk: 15/8-07)
Referanse :1.Tokuda K, Tanimoto K. New method of measuring serum bilirubin using vanadic acid. Jpn J Clin Chem. 1993;22:116-122.
2.Tietz Fundamentals of Clinical Chemistry. 5th ed. Burtis CA, Ashwood ER, eds. Philadelphia, PA: WB Saunders Company; 2001:605.
3.Young DS. Effects of Drugs on Clinical Laboratory Tests. 3rd ed. Washington: AACC Press (1990).
4.Clinical and Laboratory Standards Institute (formerly NCCLS). Evaluation of Precision Performance of Quantitative Measurement Methods; Approved Guideline – Second Edition. Wayne, PA: Clinical and Laboratory Standards Institute; 2004. NCCLS Document EP05-A2.
5.Tietz NW. Clinical Guide to Laboratory Tests. 3rd ed. Philadelphia, PA: WB Saunders Company; 1995:88-91.
6.Study Group on Bilirubin for the Committee on Standards of the American
Association for Clinical Chemistry and the National Reference System for the Clinical Laboratory. NCCLS; Dec. 1986.

B-hydroksybutarat (HBA)

Engelsk navn:ß-hydroxybutarate
Analyseinstrument:Advia®1800
Reagensprodusent:Randox
Analyseprinsipp:Enzymatic / NAD+ – NADH
This kinetic enzymatic method is based on the oxidation of ß-hydroxybutarate to acetoacetate by the enzyme ß-hydroxybutarate dehydrogenase. Concomitant with this oxidation the cofactor NAD+ is reduced to NADH. The change in absorbance at 340 nm can be directly correlated with the ß-hydroxybutyrate concentration.(Metoden tatt i bruk: 1/7-01)

Aspartat aminotransferase (AST)

Engelsk navn:Aspartate aminotransferase
Analyseinstrument:Advia®1800
Reagensprodusent:Siemens Medical Solutions Diagnostics
Analyseprinsipp:Modified IFCC
The concentration of NADH is measured by its absorbance at 340 nm, and the rate of absorbance decrease is proportional to the AST activity. The reaction is initiated by the addition of α-ketoglutarate as a second reagent.
Metoden tatt i bruk: 01.07.01
Referanse :1.International Federation of Clinical Chemistry, Committee on Standards, J Clin Chem Clin Biochem 18:521-534 (1980)
2.Tietz, NW: Clinical Guide to Laboratory Tests, 3rd Edition WB Saunders Company, Philadelphia, PA pp 76-77 (1995)