Category Archives: Metoder

Alkalisk fosfatase (AP)

Engelsk navn:Alkaline phosphatase
Analyseinstrument:Advia®1800
Reagensprodusent:Siemens Medical Solutions Diagnostics
Analyseprinsipp:Modifisert IFCC
The alkaline phosphatase hydrolyses the PNPP substrate to form
p-nitrophenol that is coloured and provides its own chromogen. The reaction is followed by the colorimetric measurement of the rate of formation of p-nitrophenol at 410 nm, which is proportional to the alkaline phosphatase activity.
Metoden tatt i bruk: 24.04.05
Referanse :1.Alkaline Phosphatase Study Group. Committee on Standards of the AACC, Subcommittee on Enzymes, Tietz NW (chairman) et al. Progress in the development of a recommended method for alkaline phosphatase activity measurement. Clin Chem 26(7):1023 (1980)
2.Tietz, NW: Clinical Guide to Laboratory Tests, 3rd Edition
WB Saunders Company, Philadelphia, PA pp 30-33 (1995)

Amylase (Amy)

Engelsk navn:Amylase
Analyseinstrument:Advia®1800
Reagensprodusent:Siemens Medical Solutions Diagnostics
Analyseprinsipp:Ethylidene Blocked-pNPG7
This rate method uses ethylidene blocked p-nitrophenyl-maltoheptaoside as substrate. The indicator enzyme α-glucosidase, used to release the p-nitrophenol, is also employed in the method. The terminal glucose of the substrate is chemically blocked preventing cleavage by the indicator enzymes and is measured at 410 nm.
Metoden tatt i bruk: 01.07.01
Referanse :1.Jensen AP and Wydeveld A: α-(p-nitrophenyl)malto hexaoside as a substrate for the assay of amylase.
Nature 182:525-526 (1958)
2.Tietz, NW: Clinical Guide to Laboratory Tests, 3rd Edition
WB Saunders Company, Philadelphia, PA pp 46-49 (1995)
3.Tietz, NW: Clinical Guide to Laboratory Tests, 2nd Edition
WB Saunders Company, Philadelphia, PA pp 52 (1990).

Ammoniakk (NH3)

Engelsk navn:Ammonia
Analyseinstrument:Advia®1800
Reagensprodusent:Roche
Analyseprinsipp :NADPH – NADP+Enzymatic
Ammonia reacts with α-ketoglutarate and NADPH to form glutamate and NADP+. The amount of NADPH oxidized during the reaction is equivalent to the amount of ammonia in the specimen and can be measured photometrically by the resulting decrease in absorbance at 340nm. The reaction is catalysed by glutamate dehydrogenase (GLDH).
Metoden tatt i bruk: 01.07.01
Referanse :1.Berthelot MPE: Repert Chem Appl. 1859:282
2.Kirsten E et al: Biochem Z 337:312 (1963)
3.Da Fonseca-Wollheim F: Z Klin Chem Klin Biochem.
11:421,426 (1973)
4.Siegel JM, Montgomery GA: Arch Biochem/Biophys 82:288 (1956)
5.Hohorst HJ: Biochem Z 328:507 (1956)
6.Tietz, NW: Clinical Guide to Laboratory Tests, 3rd Edition
WB Sauders Company, Philadelphia, PA p 44 (1995)
7.Kaplan LA, Pesce AJ: Clinical Chemistry Theory, Analysis and Correlation, CV Mosby Co, St.Louis Mo p1231 (1984)
8.Data on file at Roche Diagnostics
9.Prellwitz W et al: Med Welt 27:1277 (1976)
10.Passing H, Bablok W: A New Biometrical Procedure for Testing the Equality of Measurements from Two Different Analytical Methods.
J Clin Chem Clin Biochem 21:709-720(1983)
11.Bablok W et al: A General Regression Procedure for Method Transformation. J Clin Chem Clin Biochem 26:783-790 (1988)

Alaninminotransferase (ALT)

Engelsk navn:Alanine aminotransferase
Analyseinstrument:Advia®1800
Reagensprodusent:Siemens Medical Solutions Diagnostics
Analyseprinsipp:Modified IFCC
The reaction is initiated by the addition of α-ketoglutarat as a second reagent. The concentration of NADH is measured by its absorbance at 340 nm, and the rate of absorbance decrease is proportional to the ALT activity.
Metoden tatt i bruk: 01.07.01
Referanse :1.International Federation of Clinical Chemistry, Committee on Standards, J Clin Chem Clin Biochem 18:521-534 (1980)
2.Tietz, NW: Clinical Guide to Laboratory Tests, 3rd Edition
WB Saunders Company, Philadelphia, PA pp 20-21 (1995)

Albumin (Alb)

Engelsk navn:Albumin
Analyseinstrument:Advia®1800
Reagensprodusent:Siemens Medical Solutions Diagnostics
Analyseprinsipp:BCG (bromocresol green)
Serum albumin quantitatively binds albumin to BCG to form an albumin-BCG complex that is measured as an endpoint reaction
at 596 nm.
Metoden tatt i bruk: 01.07.01
Referanse:1.Doumas BT and Biggs HG: Determination of serum albumin.
In Standard Methods of Clinical Chemistry. GA Cooper, ed, Academic Press, Inc., New York, Vol. 7, p 175 (1972)
2.Tietz, NW: Clinical Guide to Laboratory Tests, 3rd Edition
WB Saunders Company, Philadelphia, PA pp 22-23 (1995)