Gallesyrer (GS)

Engelsk navn:Total Bile Acids
Analyseprinsipp:Enzymatic amplification / Thio-NAD – Thio-NADH
In the presence of Thio-NAD, the enzyme 3-α hydroxysteroid dehydrogenase (3-α HSD) converts bile acids to 3-keto steroids and Thio-NADH. The reaction is reversible and 3-α HSD can convert 3-keto steroids and Thio-NADH to bile acids and Thio-NAD. In the presence of excess NADH, the enzyme cycling occurs efficiently and the rate of formation of Thio-NADH is determined by measuring specific change of absorbance at 410nm.(Metoden tatt i bruk: 18/8-05)