Gamma-glutamyl transferase (GGT)

Engelsk navn:Gamma-glutamyl transferase
Reagensprodusent:Siemens Medical Solutions Diagnostics
Analyseprinsipp:Modified IFCC
In the reaction with synthetic substrate (L-γ-glutamyl-3-carboxy-4-nitroanilide), glycylglycine acts as an acceptor for the gamma-glutamyl residue and 5-amino-2-nitro-benzoate (ANB) is liberated. The liberated product has an absorption maximum near 400 nm; the rate of formation is measured photometrically at 410 nm as a zero-order kinetic assay.(Metoden tatt i bruk: 1/7-01)
Referanse :1.L.M. Shaw, J.H. Strømme, J.L. Loudon, and L. Theodosen: IFCC Methods for the Measurement of Catalytic Concentration of Enzymes. Part 4 IFCC Method for γ-Glutamyltransferase. J.Clin Chem. Biochem 21:633 – 646 (1983)
2.Tietz, NW: Clinical Guide to Laboratory Tests, 3rd Edition WB Saunders Company, Philadelphia, PA pp 286-287 (1995)