Glukose (Glu)
| Engelsk navn: | Glucose | |
| Analyseinstrument: | Advia®1800 | |
| Reagensprodusent: | Siemens Medical Solutions Diagnostics | |
| Analyseprinsipp: | Hexokinase The glucose is phosphorylated by adenosine triphosphate (ATP) in the presence of hexokinase. The glucose-6- phosphate that forms is oxidized in the presence of glucose-6-phosphate dehydrogenase causing the reduction of NAD to NADH. The absorbance of NADH is measured as an endpoint reaction at 340 nm. (Metoden tatt i bruk: 25/11-02) | |
| Referanse : | 1. | Slein MW: Methods of Enzymatic Analysis. Bergmeyer HU, ed. Academic Press, New York NY pp 1196-1201 (1974) |
| 2. | Slein MW, Cori GT and Cori CF: A comparative study of hexokinase from yeast and animal tissues. J Biol Chem 186: 763-780 (1950) | |
| 3. | Tietz, NW: Clinical Guide to Laboratory Tests, 3rd Edition WB Saunders Company, Philadelphia, PA pp 268-269 (1995) | |