Glukose (Glu)

Engelsk navn:Glucose
Reagensprodusent:Siemens Medical Solutions Diagnostics
The glucose is phosphorylated by adenosine triphosphate (ATP) in the presence of hexokinase. The glucose-6- phosphate that forms is oxidized in the presence of glucose-6-phosphate dehydrogenase causing the reduction of NAD to NADH. The absorbance of NADH is measured as an endpoint reaction at 340 nm.
(Metoden tatt i bruk: 25/11-02)
Referanse :1.Slein MW: Methods of Enzymatic Analysis. Bergmeyer HU, ed.
Academic Press, New York NY pp 1196-1201 (1974)
2.Slein MW, Cori GT and Cori CF: A comparative study of hexokinase from yeast and animal tissues.
J Biol Chem 186: 763-780 (1950)
3.Tietz, NW: Clinical Guide to Laboratory Tests, 3rd Edition
WB Saunders Company, Philadelphia, PA pp 268-269 (1995)