Jern (Fe)
Engelsk navn: | Iron | |
Analyseinstrument: | Advia®1800 | |
Reagensprodusent: | Siemens Medical Solutions Diagnostics | |
Analyseprinsipp: | Ferrozine Ferric iron is dissociated from its carrier protein, transferrin, in an acid medium and simultaneously reduced to the ferrous form. The ferrous iron is then complexed with the ferrozine, a sensitive iron indicator, to produce a colored chromophore which absorbs maximally at 571 nm.(Metoden tatt i bruk: 1/7-01) | |
Referanse: | 1. | Artiss JD, Vinogradov S and Zak B: Spectrophotometric study of several sensitivity reagents for serum iron. Clin Biochem 14(6): 311-315 (1981) |
2. | Siedel J: Improved Ferrozine® based reagent for the determination of serum iron (transferrin iron) without deproteinization. Clin Chem 30(6): 975 (1984) | |
3. | Young DS, Pestaner LC, Gibberman V. Effect of drugs on clinical laboratory tests, Clin Chem. 1975; 21:5:1D-432D. | |
4. | Tietz, NW: Clinical Guide to Laboratory Tests, 3rd Edition WB Saunders Company, Philadelphia, PA p.374 (1995) | |
5. | National Committee for Clinical Laboratory Standards. Precision performance of clinical chemistry devices; Approved Guideline-Second Edition. Wayne, PA: NCCLS; 1999. NCCLS Document EP5-A. |