|Reagensprodusent:||Siemens Medical Solutions Diagnostics|
The cholesterol esters are hydrolysed by cholesterol esterase to cholesterol and free fatty acids. The cholesterol is converted to cholesterol-3-one by cholesterol oxidase in the presence of oxygen to form hydrogen peroxide. A coloured complex is formed from hydrogen peroxidase, 4-aminoantipyrine and phenol under the catalytic influence of peroxidase. The absorbance of the complex is measured as an endpoint reaction at 505 nm.
(Metoden tatt i bruk: 1/7-01)
|Referanse:||1.||Richmond W: Preparation and properties of a cholesterol oxidase from Nocardia sp. And its application to the enzymatic assay of total cholesterol in serum. Clin Chem 19: 1350-1356 (1973)|
|2.||Roeschlau P, Bernt E and Gruber WJ: Enzymatic determination of total cholesterol in serum. Clin Chem Clin Biochem 12(5): 226 (1974)|
|3.||Allain CC, Poon LS, Chan CSG, Richmond W and Fu PC:|
Enzymatic determination of total serum cholesterol.
Clin Chem 20: 470 (1974)
|4.||Trinder P: Determination of glucose in blood using glucose oxidase with an alternative oxygen acceptor. Ann Clin Biochem 6: 24-27 (1969)|
|5.||National Cholesterol Education Program. Report of expert panel detection, evaluation and treatment of high blood cholesterol in adults. National Heart, Lung and Blood Institute, Bethesda, MD 20892. Arch Int Med 148: 36-69 (1988)|