Laktat dehydrogenase (LD)

Engelsk navn:Lactate Dehydrogenase
Analyseinstrument:Advia®1800
Reagensprodusent:Siemens Medical Solutions Diagnostics
Analyseprinsipp:Enzymatio (L-P) / TRIS / NAD+-NADH
LD catalyses the conversion of L-Lactate to pyruvate in the presence of NAD (nicotinamide adenine dinucleotide). The enzymatic activity of LD is proportional to the rate of production of NADH (reduced NAD). The amount of NADH produced is determined by measuring the increase of absorbance at 340 nm.
(Metoden tatt i bruk: 26/04-05)
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Clin Chem 21: 1018 (1975)
3.Wahlenfield AW: UV-method with L-lactate and NAD. In: Methods of Enzymatic Analysis, Volume III, 3rd edition HU Bergmeyer, ed, Verlag Chemie, Weinheim (1983)
4.Tietz NW: Textbook of Clinical Chemistry.
WB Saunders Company, Philadelphia, PA p 710 (1990)
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AM J Clin Pathol 61: 108-113 (1974)
6.Tietz, NW: Clinical Guide to Laboratory Tests, 3rd Edition
WB Saunders Company, Philadelphia, PA pp 384-385 (1995)