Laktat dehydrogenase (LD)
| Engelsk navn: | Lactate Dehydrogenase | |
| Analyseinstrument: | Advia®1800 | |
| Reagensprodusent: | Siemens Medical Solutions Diagnostics | |
| Analyseprinsipp: | Enzymatio (L-P) / TRIS / NAD+-NADH LD catalyses the conversion of L-Lactate to pyruvate in the presence of NAD (nicotinamide adenine dinucleotide). The enzymatic activity of LD is proportional to the rate of production of NADH (reduced NAD). The amount of NADH produced is determined by measuring the increase of absorbance at 340 nm. (Metoden tatt i bruk: 26/04-05) | |
| Referanse: | 1. | Amador E, Dorfman LE and Wacker WE : Serum lactic dehydrogenase activity: an analytical assessment of current assays. Clin Chem 9: 391 (1963) |
| 2. | Richards AH, Lubibski RM and Vanderlinde RE: Studies on the kinetic assay of lactate dehydrogenase activity. Clin Chem 21: 1018 (1975) | |
| 3. | Wahlenfield AW: UV-method with L-lactate and NAD. In: Methods of Enzymatic Analysis, Volume III, 3rd edition HU Bergmeyer, ed, Verlag Chemie, Weinheim (1983) | |
| 4. | Tietz NW: Textbook of Clinical Chemistry. WB Saunders Company, Philadelphia, PA p 710 (1990) | |
| 5. | Lum G and Gambino SR: A comparison of serum versus heparinized plasma for routine laboratory tests. AM J Clin Pathol 61: 108-113 (1974) | |
| 6. | Tietz, NW: Clinical Guide to Laboratory Tests, 3rd Edition WB Saunders Company, Philadelphia, PA pp 384-385 (1995) | |