Laktat (Lakt)

Engelsk navn:Lactate
Analyseinstrument:Advia®1800
Reagensprodusent:Roche
Analyseprinsipp:Enzymatic/Trinder
First, lactate is converted to pyruvate in an enzymatic reaction. The hydrogen peroxide produced by this reaction is then used in an enzymatic reaction to generate a coloured dye. The intensity of the colour formed is proportional to the L-lactate concentration and is measured as an endpoint reaction at 658 nm.
Referanse:1.Gutmann I, Wahlefeld A : Methods of Enzymatic Analysis.
2nd ed. Bergmeyer HU (ed). Academic Press Inc, New York NY 1464 (1974).
2.Noll F : Methods of Enzymatic Analysis. 2nd ed. Bergmeyer HU (ed). Academic Press Inc, New York NY 1475 (1974).
3.Trinder P: Determination of glucose in blood using glucose oxidase with an alternative oxygen acceptor. Ann Clin Biochem 6: 24(1969).
4.4. Barhan D, Trinder P : An improved colour reagent for the
determination of blood glucose by the oxidase system. Analyst 97:142 (1972)
5.Tietz, NW: Clinical Guide to Laboratory Tests, 3rd Edition
WB Saunders Company, Philadelphia, PA pp 382-383 (1995)
6.Burtis CA, Ashwood ER, eds. Tietz Textbook of Clinical
Chemistry. 2nd ed. WB Saunders Company, Philadelphia, PA p 976 (1994).
7.Westgard JO, Lahmeyer BL, Birnbaum ML : Clin Chem. 18: 1334-1338 (1972).
8.Data on file at Roche Diagnostics.
9.Passing H, Bablok W: A New Biometrical Procedure for
Testing the Equality of Measurements from Two Different
Analytical Methods: J Clin Chem Clin Biochem 21: 709-720 (1983)
10.Bablok W et al: A General Regression Procedure for Method
Transformation: J Clin Chem Clin Biochem 26: 783-790 (1988)