First, lactate is converted to pyruvate in an enzymatic reaction. The hydrogen peroxide produced by this reaction is then used in an enzymatic reaction to generate a coloured dye. The intensity of the colour formed is proportional to the L-lactate concentration and is measured as an endpoint reaction at 658 nm.
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2nd ed. Bergmeyer HU (ed). Academic Press Inc, New York NY 1464 (1974).
|2.||Noll F : Methods of Enzymatic Analysis. 2nd ed. Bergmeyer HU (ed). Academic Press Inc, New York NY 1475 (1974).|
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|4.||4. Barhan D, Trinder P : An improved colour reagent for the|
determination of blood glucose by the oxidase system. Analyst 97:142 (1972)
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|8.||Data on file at Roche Diagnostics.|
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