Lipase (Lip)

Engelsk navn:Lipase
Reagensprodusent:Siemens Medical Solutions Diagnostics
Analyseprinsipp:Colorimetric rate
The chromogenic lipase substrate, DGGMR, (1,2-o-dilauryl-rac-glycero-3-glutaric acid-(6′-methylresorufin) ester) is cleaved by the catalytic action of lipase to form 1,2-o-dilauryl-rac-glycerol and an unstable intermediate, glutaric acid-(6′-methyl resorufin) ester. This decomposes spontaneously in an alkaline solution to form glutaric acid and methylresorufin. The lipase activity in the specimen is proportional to the production of methylresorufin in the reaction and is determined spectrophotometrically at 571 nm.
(Metoden tatt i bruk: 12.01.10)
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