Lipase (Lip)
| Engelsk navn: | Lipase | |
| Analyseinstrument: | Advia®1800 | |
| Reagensprodusent: | Siemens Medical Solutions Diagnostics | |
| Analyseprinsipp: | Colorimetric rate The chromogenic lipase substrate, DGGMR, (1,2-o-dilauryl-rac-glycero-3-glutaric acid-(6′-methylresorufin) ester) is cleaved by the catalytic action of lipase to form 1,2-o-dilauryl-rac-glycerol and an unstable intermediate, glutaric acid-(6′-methyl resorufin) ester. This decomposes spontaneously in an alkaline solution to form glutaric acid and methylresorufin. The lipase activity in the specimen is proportional to the production of methylresorufin in the reaction and is determined spectrophotometrically at 571 nm. (Metoden tatt i bruk: 12.01.10) | |
| Referanse: | 1. | Tietz NW. Lipase in serum-the elusive enzyme: An overview. Clin Chem 39:746-756 (1993). |
| 2. | Steinberg WM, Goldstein SS, Davies ND, et al. Diagnostic assays in acute pancreatitis. (Review). Ann Intern Med 102:576-580 (1985). | |
| 3. | Leybold A, Junge W. Importance of colipase for the measurement of serum lipase activity. Adv clin Enzymol 4:60-67 (1986). | |
| 4. | Young, D.S., Effects of Drugs on Clinical Laboratory Tests, AACC Press, Washington, Third Edition, 1990. | |
| 5. | Clinical and Laboratory Standards Institute (formerly NCCLS). Evaluation of Precision Performance of Quantitative Measurement Methods; Approved Guideline – Second Edition. Wayne, PA: Clinical and Laboratory Standards Institute; 2004. NCCLS Document EP05-A2. | |