Urea (Urea)

Engelsk navn:Urea Nitrogen
Reagensprodusent:Siemens Medical Solutions Diagnostics
Analyseprinsipp:Enzymatic: urease with GLDH
The urea is hydrolysed in the presence of water and urease to produce ammonia and carbon dioxide. The ammonia reacts with 2-oxoglutarate in the presence of glutamate dehydrogenase and NADH. The oxidation of NADH to NAD is measured as an inverse rate reaction at 340 nm.
(Metoden tatt i bruk: 1/7-01)
Referanse:1.Roch-Ramel F : An enzymatic and fluorophotometric method for estimating urea concentrations in nanoliter specimens.
Anal Biochem 21: 372-381 (1967
2.Tietz, NW: Clinical Guide to Laboratory Tests, 3rd Edition
WB Saunders Company, Philadelphia, PA pp 622-624 (1995)