Urea (Urea)
| Engelsk navn: | Urea Nitrogen | |
| Analyseinstrument: | Advia®1800 | |
| Reagensprodusent: | Siemens Medical Solutions Diagnostics | |
| Analyseprinsipp: | Enzymatic: urease with GLDH The urea is hydrolysed in the presence of water and urease to produce ammonia and carbon dioxide. The ammonia reacts with 2-oxoglutarate in the presence of glutamate dehydrogenase and NADH. The oxidation of NADH to NAD is measured as an inverse rate reaction at 340 nm. (Metoden tatt i bruk: 1/7-01) | |
| Referanse: | 1. | Roch-Ramel F : An enzymatic and fluorophotometric method for estimating urea concentrations in nanoliter specimens. Anal Biochem 21: 372-381 (1967 |
| 2. | Tietz, NW: Clinical Guide to Laboratory Tests, 3rd Edition WB Saunders Company, Philadelphia, PA pp 622-624 (1995) | |