Urin, total protein (U-protein)

Engelsk navn:Urine, total protein
Reagensprodusent:Siemens Healthcare Diagnostics Inc.
Analyseprinsipp:This method is an endpoint chemistry. It is based on the measurement of the shift in absorption that occurs when pyrogallol red-molybdate complex binds with protonated basic amino groups of proteins at an acidic pH. The increase in absorbance at 596/694 nm is proportional to protein concentration in the sample.
Metoden tatt i bruk: 28.02.08
Referanse:1.Tietz NW. Clinical Guide to Laboratory Tests. 3rd ed. Philadelphia, PA: WB Saunders Company; 1995:520-521.
2.Fujita Y, Mori I, Kitano S. Color Reaction between Pyrogallol Red-Molybdenum Complex and Protein. Bunseki Kagaku 1983;32:E379-E386.
3.Orsonneau JL, Douet P, Massoubre C, et al. An improved pyrogallol red-molybdate method for determining total urinary protein. Clin Chem 1989;35(11):2233-2236.
4.Henry RJ, Cannon DC, Winkelman JW. Clinical Chemistry, Principles and Techniques. 2nd ed. Harper & Row; 1974.
5.Young DS. Effects of Drugs on Clinical Laboratory Tests. 5th ed. Washington, DC:AACC Press; 2000.
6.Clinical and Laboratory Standards Institute (formerly NCCLS). Evaluation of Precision Performance of Quantitative Measurement Methods; Approved Guideline – Second Edition. Wayne, PA: Clinical and Laboratory Standards Institute; 2004. NCCLS Document EP05-A2.