Urin, total protein (U-protein)
| Engelsk navn: | Urine, total protein | |
| Analyseinstrument: | ADVIA®1800 | |
| Reagensprodusent: | Siemens Healthcare Diagnostics Inc. | |
| Analyseprinsipp: | This method is an endpoint chemistry. It is based on the measurement of the shift in absorption that occurs when pyrogallol red-molybdate complex binds with protonated basic amino groups of proteins at an acidic pH. The increase in absorbance at 596/694 nm is proportional to protein concentration in the sample. Metoden tatt i bruk: 28.02.08 | |
| Referanse: | 1. | Tietz NW. Clinical Guide to Laboratory Tests. 3rd ed. Philadelphia, PA: WB Saunders Company; 1995:520-521. |
| 2. | Fujita Y, Mori I, Kitano S. Color Reaction between Pyrogallol Red-Molybdenum Complex and Protein. Bunseki Kagaku 1983;32:E379-E386. | |
| 3. | Orsonneau JL, Douet P, Massoubre C, et al. An improved pyrogallol red-molybdate method for determining total urinary protein. Clin Chem 1989;35(11):2233-2236. | |
| 4. | Henry RJ, Cannon DC, Winkelman JW. Clinical Chemistry, Principles and Techniques. 2nd ed. Harper & Row; 1974. | |
| 5. | Young DS. Effects of Drugs on Clinical Laboratory Tests. 5th ed. Washington, DC:AACC Press; 2000. | |
| 6. | Clinical and Laboratory Standards Institute (formerly NCCLS). Evaluation of Precision Performance of Quantitative Measurement Methods; Approved Guideline – Second Edition. Wayne, PA: Clinical and Laboratory Standards Institute; 2004. NCCLS Document EP05-A2. | |