Urinsyre (UA)

Engelsk navn:Uric Acid
Reagensprodusent:Siemens Healthcare Diagnostics Inc.
The uric acid is converted by uricase to allantoin and hydrogen peroxide. A coloured complex is formed from hydrogen peroxide, 4-aminophenazone and TOOS [N-ethyl-N-(2-hydroxy-3-sulfopropyl)-3-methylaniline] under the catalytic influence of peroxidase. The abosorbance of the complex is measured as an endpoint reaction at 545 nm.
Metoden tatt i bruk: 01.07.01
Referanse:1.Fossati P, Prencipe L and Berti G : Use of 3,5-dichloro-2-hydroxybenzenesulfonic acid / 4-aminophenazone chromogenic system in direct enzymic assay of uric acid in serum and urine.
Clin Chem 26(2): 227-231 (1980)
2.Trinder P: Determination of glucose in blood using glucose oxidase-peroxidase system with a non-carcinogenic chromogen.
J Clin Pathol 22: 158-161 (1969)
3.Tietz, NW: Clinical Guide to Laboratory Tests, 3rdEdition
WB Saunders Company, Philadelphia, PA pp 624-625 (1995)